Original Research

Comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces

M. De Beer, I. Peenze, V.M. da Costa Mendes, A.D. Steele
Journal of the South African Veterinary Association | Vol 68, No 3 | a883 | DOI: https://doi.org/10.4102/jsava.v68i3.883 | © 1997 M. De Beer, I. Peenze, V.M. da Costa Mendes, A.D. Steele | This work is licensed under CC Attribution 4.0
Submitted: 13 July 1997 | Published: 13 July 1997

About the author(s)

M. De Beer,
I. Peenze,
V.M. da Costa Mendes,
A.D. Steele,

Full Text:

PDF (152KB)

Abstract

The performance characteristics of 2 enzyme immunoassays (ELISAs) and 4 latex agglutination assays (LXs) were evaluated for the detection of bovine rotavirus in faecal specimens of young calves with diarrhoea. A total of 26 specimens from calves less than 5 months of age were examined with different commercial assays and compared with electron microscopy (EM) as the gold standard and with polyacrylamide gel electrophoresis (PAGE) for the detection of atypical, non-group A rotaviruses. In the 2nd study, EIA (Dako) and LX (Murex), the assays of choice, were used to analyse 97 further faecal specimens from calves with diarrhoea. The ELISAs proved to be the most sensitive compared with the other tests used. The EM and PAGE are 100 % specific although slightly less sensitive than the commercial assays. The results show that all the commercial assays can accurately detect rotavirus in the stools of calves with gastroenteritis, although the suitability and choice of assay will depend upon the requirements of individual laboratories.

Keywords

Bovine; Calf; Diarrhoea; Electron Microscopy; ELISA; Latex Agglutination; Rotavirus

Metrics

Total abstract views: 3212
Total article views: 2870

 

Crossref Citations

1. Establishment of an Indirect ELISA Method for the Detection of the Bovine Rotavirus VP6 Protein
Xiaoxia Niu, Qiang Liu, Pu Wang, Gang Zhang, Lingling Jiang, Sinong Zhang, Jin Zeng, Yongtao Yu, Yujiong Wang, Yong Li
Animals  vol: 14  issue: 2  first page: 271  year: 2024  
doi: 10.3390/ani14020271

2. Evaluation of a Human Group A Rotavirus Assay for On-Site Detection of Bovine Rotavirus
Roger K. Maes, Daniel L. Grooms, Annabel G. Wise, Cunqin Han, Valerie Ciesicki, Lora Hanson, Mary Lynne Vickers, Charles Kanitz, Robert Holland
Journal of Clinical Microbiology  vol: 41  issue: 1  first page: 290  year: 2003  
doi: 10.1128/JCM.41.1.290-294.2003

3. Molecular study of astrovirus, adenovirus and norovirus in community acquired diarrhea in children: One Egyptian center study
Maysaa El Sayed Zaki, Nermen Abo El Kheir
Asian Pacific Journal of Tropical Biomedicine  vol: 7  issue: 11  first page: 987  year: 2017  
doi: 10.1016/j.apjtb.2017.10.003

4. Diagnosis of bovine rotavirus: an overview of currently available methods
Ying Huang, Zhi Li, Yong Fu, Shu Qin Wang, Ming Kang, Ru Meng
Frontiers in Microbiology  vol: 16  year: 2025  
doi: 10.3389/fmicb.2025.1550601

5. Rotavirus
Mathew D. Esona, Rashi Gautam
Clinics in Laboratory Medicine  vol: 35  issue: 2  first page: 363  year: 2015  
doi: 10.1016/j.cll.2015.02.012

6. VP7 and VP4 Genotyping of Human Group A Rotavirus in Buenos Aires, Argentina
M. H. Argüelles, G. A. Villegas, A. Castello, A. Abrami, P. D. Ghiringhelli, L. Semorile, G. Glikmann
Journal of Clinical Microbiology  vol: 38  issue: 1  first page: 252  year: 2000  
doi: 10.1128/JCM.38.1.252-259.2000

7. Development, Evaluation, and Application of Lateral-Flow Immunoassay (Immunochromatography) for Detection of Rotavirus in Bovine Fecal Samples
Yousif Al-Yousif, Joe Anderson, Cindy Chard-Bergstrom, Sanjay Kapil
Clinical and Vaccine Immunology  vol: 9  issue: 3  first page: 723  year: 2002  
doi: 10.1128/CDLI.9.3.723-724.2002

8. A rapid method to produce high yields of purified rotavirus particles
Guillermo Adolfo Villegas, Marcelo Horacio Argüelles, Alejandro Andrés Castello, Neus Jiménez Mas, Graciela Glikmann
Journal of Virological Methods  vol: 104  issue: 1  first page: 9  year: 2002  
doi: 10.1016/S0166-0934(02)00020-4

9. Evaluation of a Latex Agglutination Kit (Virogen Rotatest) for Detection of Bovine Rotavirus in Fecal Samples
Yousif Al-Yousif, Joe Anderson, Cindy Chard-Bergstrom, Adrian Bustamante, Margaret Muenzenberger, Kimberly Austin, Sanjay Kapil
Clinical Diagnostic Laboratory Immunology  vol: 8  issue: 3  first page: 496  year: 2001  
doi: 10.1128/CDLI.8.3.496-498.2001