Original Research

Lack of susceptibility of Ehrlichia canis to imidocarb dipropionate in vitro

P.J. Kelly, L.A. Matthewman, P. Brouqui, D. Raoult
Journal of the South African Veterinary Association | Vol 69, No 2 | a815 | DOI: https://doi.org/10.4102/jsava.v69i2.815 | © 1998 P.J. Kelly, L.A. Matthewman, P. Brouqui, D. Raoult | This work is licensed under CC Attribution 4.0
Submitted: 12 July 1998 | Published: 12 July 1998

About the author(s)

P.J. Kelly,
L.A. Matthewman,
P. Brouqui,
D. Raoult,

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Abstract

In vitro antimicrobial susceptibility testing was used to compare the efficacy of imidocarb dipropionate and doxycycline on the growth of Ehrlichia canis in DH82 cell cultures. Over a 9-day period there were no significant differences (p < 0.01) in the growth of E. canis in untreated control wells and those to which imidocarb dipropionate was added at 1.2, 2.4, 4.8 or 12 mg/mℓ for the 1st 3 days. Average infection rates rose from 50 to 55 % on day 0 to 100 % on day 5 or 6. Doxycycline at 1 mg/mℓ had residual or rickettsiocidal activity against E. canis with the average percentages of DH82 cells infected declining from 51 to 24 % while the organism was exposed to the drug (3 days) and from 21 to 2 % in the 6 days following removal of the drug from the cell culture medium.

Keywords

Ehrlichia Canis; Doxycycline; Imidocarb Dipropionate; Sensitivity Testing

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