Seroepidemiological survey of Rhodococcus equi infection in asymptomatic horses and donkeys from southeast Turkey

Abstract

n order to assess the level of Rhodococcus equi infection in southeast Turkey, 679 sera from healthy foals and adult horses and 78 sera from donkeys were tested by indirect ELISA using a R. equi reference strain (ATCC 33701) as antigen. Eighty (11.7 %) sera from horses and 9 (11.5 %) sera from donkeys with titres >0.85 were positive. The prevalence of seropositive horses in Sanliurfa Province was higher than in Diyarbakir Province; 56 (13.9 %) horses in Sanliurfa Province and 24 (8.7 %) horses in Diyarbakir Province were defined as seropositive. In Sanliurfa Province 14.5 % of female (n = 343) and 10.1 % of male (n = 59) horses tested were defined as seropositive, while in Diyarbakir Province more males (11.4 %, n = 114) were seropositive than females (6.7 %, n = 163). Horses 1 to 5 years of age were found to have the highest seropositivity rate in both provinces. A total of 78 sera from donkeys were investigated in Sanliurfa Province, of which 9 (11.5 %) were positive by ELISA. Among the 9 positive sera, 6 (12.8 %) were from donkeys 1-5 years old and 3 (13.6 %) were from donkeys >5years of age. Nopositive sera were found in donkeys less than 1 year old. Five(12.5 %) sera of females and 4(10.5 %) sera of males tested were positive. These results indicate the existence of R. equi in the horse populations in Sanliurfa and Diyarbakir Provinces. Similar infection rates were found for donkeys in Sanliurfa. This suggests the importance of serological surveys to diagnose R. equi infection in the region and to prevent the zoonotic risk.