Original Research

Detection of the bovine viral diarrhoea / mucosal disease (BVD/MD) virus in tissues from aborted ruminant foetuses using immunohistochemistry

S.M. Njiro, C.M. Nkosi
Journal of the South African Veterinary Association | Vol 80, No 4 | a213 | DOI: https://doi.org/10.4102/jsava.v80i4.213 | © 2009 S.M. Njiro, C.M. Nkosi | This work is licensed under CC Attribution 4.0
Submitted: 25 May 2009 | Published: 28 May 2009

About the author(s)

S.M. Njiro,
C.M. Nkosi,

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Abstract

Various tissues from aborted ruminant foetuses were collected, fixed in formalin and embedded in paraffin wax. Sections were made and exposed to a primary monoclonal antibody against the bovine viral diarrhoea / mucosal disease (BVD/MD) virus, and subsequently to a goat anti-mouse secondary antibody conjugated to horse radish peroxidase (HRP). Diaminobenzidine (DAB) was the substrate and it released a brown pigment in the tissues on reacting with the HRP in an immunohistochemistry (IHC) procedure. Of 27 aborted foetuses, an immunoperoxidase staining reaction was observed in 1 ovine and 5 bovine foetuses. The IHC procedure located BVD/MD viral antigen in a wide variety of foetal tissues including cerebral cortical neurons, the pseudostratified columnar epithelial cells lining the bronchi, alveolar lining cells and alveolar macrophages, hepatocytes, renal tubular lining cells and the Purkinje fibres in the myocardium.

Keywords

Aborted Ruminant Foetuses; BVD/MD virus; IHC

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Crossref Citations

1. Neurological diseases of ruminant livestock in Australia. IV: viral infections
AE Kessell, JW Finnie, PA Windsor
Australian Veterinary Journal  vol: 89  issue: 9  first page: 331  year: 2011  
doi: 10.1111/j.1751-0813.2011.00817.x