Original Research

Prevalence of mixed Trypanosoma congolense infections in livestock and tsetse in KwaZulu-Natal, South Africa

K. Gillingwater, M.V. Mamabolo, P.O.A. Majiwa
Journal of the South African Veterinary Association | Vol 81, No 4 | a151 | DOI: https://doi.org/10.4102/jsava.v81i4.151 | © 2010 K. Gillingwater, M.V. Mamabolo, P.O.A. Majiwa | This work is licensed under CC Attribution 4.0
Submitted: 21 May 2010 | Published: 21 May 2010

About the author(s)

K. Gillingwater,
M.V. Mamabolo,
P.O.A. Majiwa,

Full Text:

PDF (162KB)

Abstract

Trypanosoma congolense causes the most economically important animal trypanosomosis in Africa. In South Africa, a rinderpest pandemic of the 1890s removed many host animals, resulting in the near-eradication of most tsetse species. Further suppression was achieved through spraying with dichlorodiphenyltrichloroethane (DDT); however, residual populations of Glossina austeni and G. brevipalpis remained in isolated pockets. A total of 506 of these tsetse flies were captured in the Hluhluwe-iMfolozi Park, the St Lucia Wetland Park and Boomerang commercial farm. The polymerase chain reaction (PCR) was used to determine the infection rate and frequency of mixed infections of these flies. Additionally, 473 blood samples were collected from cattle at communal diptanks and a commercial farm in the area and each one examined by the haematocrit centrifugation technique (HCT). Furthermore, buffy coats from these blood samples were spotted onto FTA Elute cards and the DNA extracted from each one tested using 3 separate PCRs. The HCT revealed the presence of trypanosomes in only 6.6 % of the blood samples; by contrast, species-specific PCR detected trypanosome DNA in 50 % of the samples. The species-specific PCR detected trypanosome DNA in 17 % of the tsetse flies, compared with the nested PCR targeting rDNA which detected trypanosome DNA in only 14 % of the samples. Over time, the transmission of Savannah-type T. congolense and Kilifi-type T. congolense as mixed infections could have an impact on disease manifestation in different hosts in the area.

Keywords

mixed infection; PCR; prevalence; Trypanosoma congolense; tsetse

Metrics

Total abstract views: 3904
Total article views: 4449

 

Crossref Citations

1. Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives
Jeanne V. Samsonova, Nikolay Yu. Saushkin, Alexander P. Osipov
Veterinary Research Communications  vol: 46  issue: 3  first page: 655  year: 2022  
doi: 10.1007/s11259-022-09957-w

2. Prevalence and Associated Risk Factors of African Animal Trypanosomiasis in Cattle in Lambwe, Kenya
Ivy Okello, Eliakunda Mafie, Gillian Eastwood, Jahashi Nzalawahe, Leonard E. G. Mboera, Samuel Onyoyo, José F. Silveira
Journal of Parasitology Research  vol: 2022  first page: 1  year: 2022  
doi: 10.1155/2022/5984376

3. A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis
Thu-Thuy Nguyen, Ngasaman Ruttayaporn, Yasuyuki Goto, Shin-ichiro Kawazu, Tatsuya Sakurai, Noboru Inoue
Parasitology Research  vol: 114  issue: 11  first page: 4319  year: 2015  
doi: 10.1007/s00436-015-4672-z

4. A systematic review and meta-analysis of trypanosome prevalence in tsetse flies
Reta D. Abdi, Getahun E. Agga, Weldegebrial G. Aregawi, Merga Bekana, Thomas Van Leeuwen, Vincent Delespaux, Luc Duchateau
BMC Veterinary Research  vol: 13  issue: 1  year: 2017  
doi: 10.1186/s12917-017-1012-9

5. A meta-analysis of the prevalence of bovine trypanosomiasis in some African countries from 2000 to 2018
F. Ebhodaghe, C. Isaac, J.A. Ohiolei
Preventive Veterinary Medicine  vol: 160  first page: 35  year: 2018  
doi: 10.1016/j.prevetmed.2018.09.018

6. An update of the tsetse fly (Diptera: Glossinidae) distribution and African animal trypanosomosis prevalence in north-eastern KwaZulu-Natal, South Africa
Chantel J. De Beer, Gert J. Venter, Karin Kappmeier Green, Johan Esterhuizen, Daniel G. De Klerk, Jerome Ntshangase, Marc J.B. Vreysen, Ronel Pienaar, Makhosazana Motloang, Lundi Ntantiso, Abdalla A. Latif
Onderstepoort J Vet Res  vol: 83  issue: 1  year: 2016  
doi: 10.4102/ojvr.v83i1.1172

7. TRYPANOSOMIASIS IN INTRODUCED SOUTHERN WHITE RHINOCEROS (CERATOTHERIUM SIMUM SIMUM) GIFTS TO EX SITU HABITAT IN AITONG, KENYA
Francis Gakuya, Richard Kock, Isaac Lekolool, Steve Mihok
Journal of Wildlife Diseases  vol: 60  issue: 4  year: 2024  
doi: 10.7589/JWD-D-24-00026

8. Molecular surveillance of Theileria parasites of livestock in Oman
Amira Al-Fahdi, Badar Alqamashoui, Salama Al-Hamidhi, Onur Kose, Mohammed H. Tageldin, Patrick Bobade, Eugene H. Johnson, Abdel-Rahim Hussain, Tulin Karagenc, Andy Tait, Brian Shiels, Huseyin Bilgin Bilgic, Hamza Babiker
Ticks and Tick-borne Diseases  vol: 8  issue: 5  first page: 741  year: 2017  
doi: 10.1016/j.ttbdis.2017.05.008

9. Virulence of Trypanosoma congolense strains isolated from cattle and African buffaloes (Syncerus caffer) in KwaZulu-Natal, South Africa
Makhosazana Y. Motloang, Justin Masumu, Ben J. Mans, Abdalla A. Latif
Onderstepoort J Vet Res  vol: 81  issue: 1  year: 2014  
doi: 10.4102/ojvr.v81i1.679

10. Vector competence of Glossina austeni and Glossina brevipalpis for Trypanosoma congolense in KwaZulu-Natal, South Africa
Makhosazana Motloang, Justin Masumu, Barend Mans, Peter Van den Bossche, Abdalla Latif
Onderstepoort J Vet Res  vol: 79  issue: 1  year: 2012  
doi: 10.4102/ojvr.v79i1.353

11. Parasitological and molecular identification of Trypanosoma species circulating in cattle and tsetse flies in Matutuine District, Maputo Province, Mozambique
Inocêncio Sigaúque, Paula Alberto Macucule, Fernando Chanisso Mulandane, Denise Ribeiro Arthur Brito, Suzana Augusta Jamal, Vincent Delespaux, Luís Carlos Bernardo Gil das Neves
Rendiconti Lincei. Scienze Fisiche e Naturali  vol: 33  issue: 3  first page: 501  year: 2022  
doi: 10.1007/s12210-022-01077-6

12. Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa
Thu-Thuy NGUYEN, Mono Sophie MOTSIRI, Moeti Oriel TAIOE, Moses Sibusiso MTSHALI, Yasuyuki GOTO, Shin-Ichiro KAWAZU, Oriel Matlhahane Molifi THEKISOE, Noboru INOUE
Journal of Veterinary Medical Science  vol: 77  issue: 2  first page: 217  year: 2015  
doi: 10.1292/jvms.14-0330

13. Development of Cathepsin L-like Real-Time PCR Assays for the Detection of African Animal Trypanosomosis (AAT) in South Africa
Samantha Mnkandla, Luis Neves, Ilse Vorster, Raksha Vasantrai Bhoora
Pathogens  vol: 11  issue: 2  first page: 136  year: 2022  
doi: 10.3390/pathogens11020136

14. African Animal Trypanosomiasis: A Systematic Review on Prevalence, Risk Factors and Drug Resistance in Sub-Saharan Africa
Ivy Okello, Eliakunda Mafie, Gillian Eastwood, Jahashi Nzalawahe, Leonard E G Mboera, Athanase Badolo
Journal of Medical Entomology  vol: 59  issue: 4  first page: 1099  year: 2022  
doi: 10.1093/jme/tjac018

15. Characterization of tabanid flies (Diptera: Tabanidae) in South Africa and Zambia and detection of protozoan parasites they are harbouring
MOETI O. TAIOE, MAKHOSAZANA Y. MOTLOANG, BONIFACE NAMANGALA, AMOS CHOTA, NTHATISI I. MOLEFE, SIMON P. MUSINGUZI, KEISUKE SUGANUMA, POLLY HAYES, TOI J. TSILO, JOHN CHAINEY, NOBORU INOUE, ORIEL M. M. THEKISOE
Parasitology  vol: 144  issue: 9  first page: 1162  year: 2017  
doi: 10.1017/S0031182017000440