Short Communication

Use of Brucella abortus species specific polymerase chain reaction assay for the diagnosis of bovine brucellosis

Songelwayo L. Chisi, Tracy Schmidt, George W. Akol, Henriette van Heerden
Journal of the South African Veterinary Association | Vol 88 | a1433 | DOI: https://doi.org/10.4102/jsava.v88i0.1433 | © 2017 Songelwayo L. Chisi, Tracy Schmidt, George W. Akol, Henriette van Heerden | This work is licensed under CC Attribution 4.0
Submitted: 10 May 2016 | Published: 27 September 2017

About the author(s)

Songelwayo L. Chisi, Department of Agriculture and Rural Development KwaZulu Natal Province, Allerton Provincial Veterinary Laboratory, South Africa
Tracy Schmidt, Department of Agriculture and Rural Development KwaZulu Natal Province, Allerton Provincial Veterinary Laboratory, South Africa
George W. Akol, Centre of Veterinary Excellence, Dohne Agricultural Development Institute; Department of Rural Development and Agrarian Reform, Stutterheim, South Africa
Henriette van Heerden, Department of Tropical Diseases, University of Pretoria, South Africa

Abstract

Serology is primarily used in the diagnosis of bovine brucellosis. Bacterial culture and isolation is the gold standard in diagnosing brucellosis but, like serology, it does not offer complete (100%) diagnostic sensitivity and specificity. Polymerase chain reaction (PCR) has been suggested to offer better specificity and sensitivity. In this study, we evaluated the performance of Brucella abortus species specific (BaSS) PCR directly from different samples in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture. The BaSS PCR had a low diagnostic sensitivity (DSe) of 70%, but was able to identify vaccine strains using abomasal fluid from aborted foetuses and detect Brucella DNA from decomposing samples. The best sample for the BaSS PCR was abomasal fluid.

Keywords

brucella abortus; polymerase chain reaction; sensitivity; base pair

Metrics

Total abstract views: 3495
Total article views: 3703

 

Crossref Citations

1. Chimeric Protein Designed by Genome-Scale Immunoinformatics Enhances Serodiagnosis of Bovine Neosporosis
Higor Sette Pereira, Ludmila Tavares e Almeida, Vitória Fernandes, Renato Lima Senra, Patrícia Pereira Fontes, Eustáquio Resende Bittar, Andréa de Oliveira Barros Ribon, Polyana Pizzi Rotta, Daniel Menezes-Souza, Joely Ferreira Figueiredo Bittar, Tiago Antônio de Oliveira Mendes, Brad Fenwick
Journal of Clinical Microbiology  vol: 58  issue: 7  year: 2020  
doi: 10.1128/JCM.01343-19

2. Characterisation of Brucella species and biovars in South Africa between 2008 and 2018 using laboratory diagnostic data
Itumeleng Matle, Betty Ledwaba, Karabelo Madiba, Lavhelesani Makhado, Kudakwashe Jambwa, Nombasa Ntushelo
Veterinary Medicine and Science  vol: 7  issue: 4  first page: 1245  year: 2021  
doi: 10.1002/vms3.483

3. Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella abortus in organs of infected cattle in southern Italy
Viviana Manzulli, Valeria Rondinone, Luigina Serrecchia, Antonio Petrella, Domenico Scaltrito, Leonardo Marino, Lorenzo Pace, Maria Luigia Prencipe, Dora Cipolletta, Mauro Nitti, Antonio Fasanella, Elena Poppa, Domenico Galante
Journal of Veterinary Research  vol: 66  issue: 4  first page: 559  year: 2022  
doi: 10.2478/jvetres-2022-0062

4. Seroprevalence and associated risk factors of Brucellosis in buffaloes in district Layyah
Muhammad Kashif, Amar Nasir, Samee Ullah, Muhammad Tarique Tunio, Arbab Sikandar, Muhammad Rizwan, Muhammad Asif, Mazhar Abbas
Buffalo Bulletin  vol: 43  issue: 3  first page: 349  year: 2024  
doi: 10.56825/bufbu.2024.4334278