Original Research

An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa

Songelwayo L. Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W. Akol, Henriette van Heerden
Journal of the South African Veterinary Association | Vol 88 | a1381 | DOI: https://doi.org/10.4102/jsava.v88i0.1381 | © 2017 Songelwayo L. Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W. Akol, Henriette van Heerden | This work is licensed under CC Attribution 4.0
Submitted: 08 January 2016 | Published: 28 February 2017

About the author(s)

Songelwayo L. Chisi, Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory, South Africa
Yoanda Marageni, Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory, South Africa
Prebashni Naidoo, Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory, South Africa
Gloria Zulu, Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory, South Africa
George W. Akol, Centre of Veterinary Excellence, Dohne Agricultural Development Institute; Department of Rural Development and Agrarian Reform, Stutterheim, South Africa
Henriette van Heerden, Department of Tropical Diseases, University of Pretoria, South Africa

Abstract

The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.

Keywords

complement fixation test; indirect enzyme linked immuno absorbent assay; competitive enzyme linked immuno absorbent assay; Rose Bengal test; serum agglutination test; brucella abortus; diagnostic sensitivity; diagnostic specificity

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