Original Research

Comparison of two culture techniques used to detect environmental contamination with Salmonella enterica in a large-animal hospital

Catriona H. Lyle, Cornelius H. Annandale, Johan Gouws, Paul S. Morley
Journal of the South African Veterinary Association | Vol 86, No 1 | a1292 | DOI: https://doi.org/10.4102/jsava.v86i1.1292 | © 2015 Catriona H. Lyle, Cornelius H. Annandale, Johan Gouws, Paul S. Morley | This work is licensed under CC Attribution 4.0
Submitted: 10 March 2015 | Published: 13 August 2015

About the author(s)

Catriona H. Lyle, Department of Companion Animal Clinical Studies, University of Pretoria, South Africa
Cornelius H. Annandale, Department of Production Animal Studies, University of Pretoria, South Africa
Johan Gouws, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
Paul S. Morley, Department of Companion Animal Clinical Studies, University of Pretoria, South Africa; College of Veterinary Medicine and Biomedical Sciences, Colorado State University, United States

Abstract

Salmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for Salmonella is an integral part of comprehensive infection control programmes in populations at risk. The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. Environmental samples were collected using household cleaning cloths that were incubated overnight in buffered peptone water (BPW). Aliquots of BPW were then processed using two different selective enrichment and culture techniques. In the first technique (TBG-RV-XLT4) samples were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. The second technique (SEL-XLD) involved incubation at 37 °C in selenite broth before plating on XLD agar. Salmonella was recovered from 49.7% (73/147) of samples using the TBG-RV-XLT4 technique, but only 10.2% (15/147) of samples using the SEL-XLD method. Fourteen samples (9.5%) were culture-positive using both methods, and 73 (49.7%) were culture-negative using both techniques. There were discordant results for 60 samples, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one sample that was only culturepositive using the SEL-XLD method. Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. Environmental contamination with Salmonella may be underestimated by certain culture techniques, which may impair efforts to control spread in veterinary hospitals.

Keywords

horse, nosocomial infection, infection control, surveillance

Metrics

Total abstract views: 2954
Total article views: 5263

 

Crossref Citations

1. One Health Perspective of Salmonella Serovars in South Africa Using Pooled Prevalence: Systematic Review and Meta-Analysis
Tsepo Ramatla, Mpho Tawana, ThankGod E. Onyiche, Kgaugelo E. Lekota, Oriel Thekisoe, Todd R. Callaway
International Journal of Microbiology  vol: 2022  first page: 1  year: 2022  
doi: 10.1155/2022/8952669