Original Research

Molecular characterisation of Mycoplasma species isolated from the genital tract of Dorper sheep in South Africa

Habu A. Kalshingi, Anna-Mari Bosman, Johan Gouws, Moritz van Vuuren
Journal of the South African Veterinary Association | Vol 86, No 1 | a1199 | DOI: https://doi.org/10.4102/jsava.v86i1.1199 | © 2015 Habu A. Kalshingi, Anna-Mari Bosman, Johan Gouws, Moritz van Vuuren | This work is licensed under CC Attribution 4.0
Submitted: 19 June 2014 | Published: 08 June 2015

About the author(s)

Habu A. Kalshingi, National Veterinary Research Institute, Vom, Nigeria; Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
Anna-Mari Bosman, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
Johan Gouws, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa
Moritz van Vuuren, Department of Veterinary Tropical Diseases, University of Pretoria, South Africa


Share this article

Bookmark and Share

Abstract

Biochemical and molecular analysis were conducted on 34 strains of Mycoplasma species isolated between 2003 and 2009 from the genital tract of clinically healthy Dorper sheep and sheep with ulcerative vulvitis and balanitis. Earlier publications identified the causative agent as Mycoplasma mycoides mycoides large colony (MmmLC) and Arcanobacterium pyogenes. The aims of the study were to characterise Mycoplasma species isolated from the genital tract of Dorper sheep with polymerase chain reaction assay, cloning and gene sequencing. Basic Local Alignment Search Tool (BLAST) results revealed six predominant Mycoplasma species: Mycoplasma arginini, Mycoplasma bovigenitalium, Arcanobacterium laidlawii, MmmLC, Mycoplasma sp. ovine/caprine serogroup II and M. canadense. Sequencing of the 34 isolates were analysed using phylogenetic methods, and 18 (50%) were identified as M. arginini with 99% – 100% similarity to M. arginini from England and Sweden. Six isolates showed 99% similarity to M. bovigenitalium strains from Turkey and Germany. Two isolates had 99% similarity to an M. sp. ovine/caprine sero group II from the United Kingdom. BLAST for two isolates revealed 99% similarity to Acholeplasma laidlawii from India, another two were 99% similar to MmmLC strain from Sweden, two showed 98% similarity to Mycoplasma sp. Usp 120 from Brazil, and two isolates have a 97% – 99% similarity to M. mm. Jcv1 strain from the United States of America. Finally, one isolate showed similarity of 99% to Mycoplasma canadense strain from Italy. The findings support the hypothesis that ulcerative vulvitis and balanitis of Dorper sheep in South Africa (SA) is a multifactorial disease with involvement of different Mycoplasma species.

Keywords

No related keywords in the metadata.

Metrics

Total abstract views: 2031
Total article views: 6360


Crossref Citations

No related citations found.